Vol 1, No 2 (2015)

Reviews

Tissue Optics and Photonics: Light-Tissue Interaction

Tuchin V.V.

Abstract

This is the second section of the review-tutorial paper describing fundamentals of tissue optics and photonics. As the first section of the paper was mostly devoted to description of biological tissue structures and their specificity related to interactions with light [1], this section 3 describes light-tissue interactions themselves that caused by tissue dispersion, scattering, and absorption properties, including light reflection and refraction, absorption, elastic, and quasi-elastic scattering. The major tissue absorbers and modes of elastic scattering, including Rayleigh and Mie scattering, will be presented.

Journal of Biomedical Photonics & Engineering. 2015;1(2):98-134
pages 98-134 views

Articles

Dendritic crystallogram images classification

Paringer R., Kupriyanov A., Ilyasova N.

Abstract

A computer classification system of dendritic crystallogram images is presented in this paper. To improve the quality of classification we use an algorithm for the informative features formation, using methods of discriminant analysis. The method for receiving an informativeness estimation was used. As basic features are seven geometric characteristic were calculated. The research confirming the efficiency of the formed features for classification of dendritic crystallogramms images was conducted by means of classification by support vector machine. Of these, was selected most informative basic five features and one new feature was formed. The error classification decreased from 0.081 to 0.061. The algorithm possesses a sufficient level of universality and may be applied to increase the informativeness of any feature set.
Journal of Biomedical Photonics & Engineering. 2015;1(2):135-138
pages 135-138 views

Zernike phase spatial filter for measuring the aberrations of the optical structures of the eye

Khonina S., Kotlyar V., Kirsh D.

Abstract

To measure directly the wavefront aberration coefficients, we propose to use the multi-order diffractive element fitted with the set of Zernike polynomials. Polynomials of lowest degree describe defocusing (ametropy) and astigmatism. Coefficients of highest degree correspond to the spherical aberration of oblique rays that occurs as a consequence of misalignment of the crystalline lens and foveola, as well as deflection at the periphery of the crystalline lens. Multi-order elements allow several tens of expansions coefficients to be measured simultaneously, which will enable to investigate insufficiently known high-order aberrations for the differentiated diagnostics of eye diseases.
Journal of Biomedical Photonics & Engineering. 2015;1(2):146-153
pages 146-153 views

Compact optical tweezer with the capability of dynamic control

Korobtsov A., Kotova S., Losevsky N., Mayorova A., Samagin S.

Abstract

The extension of capabilities towards the formation of controlled complex-shaped optical traps is demonstrated for the compact laser tweezer based on the four-channel LC modulator. The experimental results on the yeast cell manipulation, including the particles larger than 10 μm, are presented. The capture and confinement of the object with the dimensions 37 μm × 13 μm was realized by means of the use of the ellipse-shaped trap. The maximal escape velocity of this object was about 20 μm/s.
Journal of Biomedical Photonics & Engineering. 2015;1(2):154-163
pages 154-163 views

Backscattering spectroscopy for assessing skin tumor

Zakharov V.P., Timchenko P.E., Timchenko E.V., Zherdeva L.A., Kozlov S.V., Moryatov A.A.

Abstract

The results of in vitro backscattering spectral studies of 7 nevuses and 27 samples of different skin cancer are presented. The pathology type classification in the two-dimensional phase space formed by the scattering indices at the wavelengths 560 nm, 650 nm, 700 nm, and 760 nm scaled to the healthy skin scattering coefficient value is proposed. It is shown that the sensitivity and specificity of differentiation between the benign and malignant tumors exceeds 86% and 96%, respectively, and their value for selecting melanoma among other malignant tumors falls by 5-8% due to partial overlap of classes in the phase space.

Journal of Biomedical Photonics & Engineering. 2015;1(2):164-168
pages 164-168 views

The combination of optical coherence tomography and Raman spectroscopy for tissue characterization

Egodage K., Dochow S., Bocklitz T., Chernavskaia O., Matthaeus C., Schmitt M., Popp J.

Abstract

The visualization as well as characterization of diseased tissue are of vital diagnostic interest for an early diagnosis to increase patients’ survival rate. In this study we introduce an imaging device combining optical coherence tomography (OCT) and Raman spectroscopy (RS), allowing to record 2D and 3D OCT cross sectional images of bulk tissue samples, as well as the acquisition of Raman spectra from small areas of interest in order to aid the detection process with molecular information. The design of the OCT/RS imaging device consists of commercially available cage components. The probe head involves a CCD camera chip for visualization purposes and galvanic mirrors for scanning the sample in x and y directions with a scan line rate up to 76 kHz. The OCT/RS imaging approach has been successfully evaluated by investigating pork samples. OCT and Raman data were correlated and different tissue types within the samples were successfully identified and clustered separately. Finally, pork skin samples with visual defects were characterized. Overall, the presented OCT/RS device allows for recording of large morphological overview OCT images to define points of interest, which are afterwards characterized in more detail on a molecular level by means of Raman spectroscopy.

Journal of Biomedical Photonics & Engineering. 2015;1(2):169-177
pages 169-177 views


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies