Vol 1, No 1 (2015)

Year: 2015
Articles: 8
URL: https://jbpe.ssau.ru/index.php/JBPE/issue/view/122

Full Issue

Reviews

Tissue Optics and Photonics: Biological Tissue Structures

Tuchin V.V.

Abstract

This is the first section of the review-tutorial paper describing fundamentals of tissue optics and photonics mostly devoted to biological tissue structures and their specificity related to light interactions at its propagation in tissues. The next sections of the paper will describe light-tissue interactions caused by tissue dispersion, scattering, and absorption properties, including light reflection and refraction, absorption, elastic quasi-elastic and inelastic scattering. The major tissue absorbers and types of elastic scattering, including Rayleigh and Mie scattering, will be presented.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):3-21

3-21

(Eng)

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Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina E.A., Bashkatov A.N., Sinichkin Y.P., Yanina I.Y., Tuchin V.V.

Abstract

A review of specific features and methods of optical clearing and related interaction of light with biotissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular biotissues are discussed. The possibility of efficient control of the biotissue optical properties, particularly, the reduction of light scattering in biotissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes

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Journal of Biomedical Photonics & Engineering. 2015;1(1):22-58

22-58

(Eng)

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Articles

CHANGE OF OPTICAL PROPERTIES OF HAIR CELLS DURING MALIGNANT TUMOR DEVELOPMENT

Maryakhina V.S., Anenkova K.

Abstract

The results of experimental investigations on the differences of wool microstructure and its biophysical properties of healthy mice and mice with malignant tumor(s) are represented in the paper. It is shown that destruction of wool structure as well as biochemical composition change of wool cells is during pathology development. Difference is seen even when tumor is small. We have shown that the general regularity of spectral measurements for mammary and hair cells is conserved at health state and pathology present. Taking into account this, the represented data about wool structure can be used to improve the optical techniques for non-invasive diagnostics of cancer diseases at different stages of pathology.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):59-63

59-63

(Eng)

(JATS XML)

LINE OPTICAL TRAPS FORMED BY LC SLM

Korobtsov A., Kotova S., Losevsky N., Mayorova A., Samagin S.

Abstract

The methods for generation of optical traps in the form of line segments by means of liquid crystal modulators of two types are addressed in the paper, they are a multi-pixel modulator HOLOEYE HEO 1080P and a tunable liquid crystal focusing device (4-channel modulator) developed by the authors. The numerical and experimental assessment of the capture forces of the generated optical trap is fulfilled. The description of manipulation experiments with microobjects, including biological ones, carried out by the optical traps with the intensity distribution in the form of segments is offered.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):64-69

64-69

(Eng)

(JATS XML)

Lung neoplasm diagnostics using Raman spectroscopy and autofluorescence analysis

Artemyev D.N., Zakharov V.P., Bratchenko I.A., Myakinin O.O., Kornilin D.V., Kozlov S.V., Moryatov A.A.

Abstract

A method of lung neoplasm diagnostics is proposed based on the combined analysis of Raman and autofluorescence spectra excited by laser beam with the wavelength 785 nm. Such approach allows fast selection of the pathology region followed by differentiation of the neoplasm type. The developed method demonstrated the sensitivity of 90.9% and the specificity of 71.4% in the differentiation of squamous cell carcinoma and adenocarcinoma cases.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):70-76

70-76

(Eng)

(JATS XML)

OCT/LCT monitoring of drug action on the structure of the human cornea in vivo

Genina E.A., Bashkatov A.N., Kamenskikh I.D., Kolbenev I.O., Kamenskikh T.G., Tuchin V.V.

Abstract

The effect of anti-glaucoma medicinal prepaprations Timolol-AKOS and Cosopt on the structure components of human eye cornea are studied. The eye drops Timolol-AKOS 0.5% and Cosopt were used as the object of study. 10 voluntary patients of the Eye Disease Clinic aged from 70 to 75 suffering from glaucoma took part in the studies. The study of cornea in vivo was carried out using the methods of laser scanning confocal tomography (LCT) and optical coherence tomography (OCT) before the application of drugs and at different time moments after the application. The eye cornea thickness values measured using the OCT at the initial moment of time and at different times after the application of the medicinal preparations are presented. From the LCT data the density and the mean diameter of the cornea epithelial cells was calculated. As a result of the complex studies carried out, it was shown that the Timolol-AKOS medication causes the swelling of the cornea within the limits of 1-5%. Under the use of Cosopt medication the cornea dehydration was observed.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):77-80

77-80

(Eng)

(JATS XML)

A pulsed photoacoustic technique for studying red blood cell sedimentation

Kinnunen M.

Abstract

This study shows the capability of a pulsed photoacoustic (PA) technique to measure red blood cell sedimentation and aggregation processes in vitro. Red blood cells are the main source of absorption in blood. The PA signal is proportional to the sample’s optical absorption coefficient, and hence, dynamic changes in the sample can be monitored by analyzing the PA pulse amplitude and pulse arrival time. Optical coherence tomography (OCT) is used as a parallel method for comparison. Diluted whole blood and different concentrations of washed red blood cells were used as samples. The pulsed PA technique is suitable for monitoring changes in sedimentation velocity when dextran is added to the sample. When the measurement section with the fastest sedimentation rate was selected for analysis, a more than 10-fold increase in the sedimentation rate, induced by dextran, was found with both the PA and OCT techniques. The PA pulse delay was found to be a more reliable measure of changes in the sample than the PA signal amplitude.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):81-89

81-89

(Eng)

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Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero

Larin K.

Abstract

Mouse models are commonly used as research tools to understand regulatory pathways affected by human diseases and disorders. Live imaging tools for visualization of mouse embryonic ocular tissues would be beneficial in research associated with developmental ocular defects. In this study, in utero quantitative assessment of ocular structures in mouse embryos was performed with a swept-source optical coherence tomography (SSOCT). To define developmental changes in eye morphology in live embryos, the volume of the embryonic eye lens and the globe at different embryonic stages ranging from E13.5 to E18.5 was quantified. It is determined that the major axis diameter of the eye lens and the globe was found to increase from 0.44±0.18 mm to 0.98±0.05 mm and from 0.56±0.22 mm to 1.23±0.14 mm, respectively, as the embryo ages from E13.5 to E18.5. For the same stages, the volume of the eye lens and globe was found to increase from 0.028±0.027 mm³ to 0.32±0.08 mm³ and from 0.085±0.08 mm³ to 0.75±0.27 mm³, respectively. These results suggest that OCT can accurately assess developmental processes of ocular structures and can be potentially used to assess embryonic ocular growth in mouse mutants with eye abnormalities and to study the effect of toxicological and pharmacological agents.

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Journal of Biomedical Photonics & Engineering. 2015;1(1):90-95

90-95